dox treatment (Thermo Fisher)

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Thermo Fisher dox treatment

Workflow for patient-derived osteosarcoma (PDOS) organoid generation, embedding in photocrosslinkable ECMs, and analysis. (a) Method for generation of <t>PDOS</t> <t>organoids.</t> (b) On day 3 of culture, PDOS organoids were embedded in photocrosslinkable matrix precursors and crosslinked using visible light to prepare OS-matrix microenvironments. (c) Method for determination of organoid core and invasion area using brightfield images and ImageJ analysis. (d) Matrix-based response to doxorubicin <t>(DOX)</t> was used to evaluate the effect of matrix type and stiffness on OS response to chemotherapy.

Dox Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dox+treatment/Fluorescein/pmc13269220-84-19-31
Average 99 stars, based on 1 article reviews

dox treatment - by Bioz Stars, 2026-10

99/100 stars

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1) Product Images from "Matrix type influences embedded patient-derived osteosarcoma organoid invasion and response to treatment"

Article Title: Matrix type influences embedded patient-derived osteosarcoma organoid invasion and response to treatment

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2026.1831729

Figure Legend Snippet: Workflow for patient-derived osteosarcoma (PDOS) organoid generation, embedding in photocrosslinkable ECMs, and analysis. (a) Method for generation of PDOS organoids. (b) On day 3 of culture, PDOS organoids were embedded in photocrosslinkable matrix precursors and crosslinked using visible light to prepare OS-matrix microenvironments. (c) Method for determination of organoid core and invasion area using brightfield images and ImageJ analysis. (d) Matrix-based response to doxorubicin (DOX) was used to evaluate the effect of matrix type and stiffness on OS response to chemotherapy.

Techniques Used: Derivative Assay

Doxorubicin (DOX) treatment of matrix-free and embedded PDOS organoids. (a) Fluorescein diacetate (FDA)-based staining of live PDOS organoids treated with mid-concentration DOX (0.1 µM) was conducted at endpoint of treatment (day 4 post-embedding). (b) Metabolic activity of matrix-free and embedded organoids determined on day 7 of culture (day 4 post-embedding). The relative metabolic activity of each sample was determined through normalization to respective group vehicle controls. Heatmap values represent mean. N = 1. n = 6. (c) IC 50 values for PDOS cultures was determined via [inhibitor] vs. response variable slope (four parameters) non-linear regression.

Figure Legend Snippet: Doxorubicin (DOX) treatment of matrix-free and embedded PDOS organoids. (a) Fluorescein diacetate (FDA)-based staining of live PDOS organoids treated with mid-concentration DOX (0.1 µM) was conducted at endpoint of treatment (day 4 post-embedding). (b) Metabolic activity of matrix-free and embedded organoids determined on day 7 of culture (day 4 post-embedding). The relative metabolic activity of each sample was determined through normalization to respective group vehicle controls. Heatmap values represent mean. N = 1. n = 6. (c) IC 50 values for PDOS cultures was determined via [inhibitor] vs. response variable slope (four parameters) non-linear regression.

Techniques Used: Staining, Concentration Assay, Activity Assay

Morphological response of PDOS organoids to treatment with increasing DOX concentration. (a) Brightfield microscopy of PDOS organoids treated with DOX demonstrates that core and invasive areas were reduced in all groups with increasing DOX concentration. (b) Quantification of spheroid core areas revealed that core size was slightly decreased with increasing DOX concentration for most matrix types. N = 1. n = 6. Error bars = STDEV. (c) Total organoid area quantification shows that groups with high invasion, such as soft LungMA and soft GelMA, undergo a decrease in total cell area with increasing DOX concentration. N = 1. n = 6. Error bars = STDEV. (d) Quantification of the percentage of core and invasive cell areas in relation to total cell coverage acts as an indicator for drug-based invasion inhibition. N = 1. n = 6. Error bars = STDEV.

Figure Legend Snippet: Morphological response of PDOS organoids to treatment with increasing DOX concentration. (a) Brightfield microscopy of PDOS organoids treated with DOX demonstrates that core and invasive areas were reduced in all groups with increasing DOX concentration. (b) Quantification of spheroid core areas revealed that core size was slightly decreased with increasing DOX concentration for most matrix types. N = 1. n = 6. Error bars = STDEV. (c) Total organoid area quantification shows that groups with high invasion, such as soft LungMA and soft GelMA, undergo a decrease in total cell area with increasing DOX concentration. N = 1. n = 6. Error bars = STDEV. (d) Quantification of the percentage of core and invasive cell areas in relation to total cell coverage acts as an indicator for drug-based invasion inhibition. N = 1. n = 6. Error bars = STDEV.

Techniques Used: Concentration Assay, Microscopy, Inhibition

Microarray:

Article Title: Refined culture conditions with increased physiological relevance uncover oncogene-dependent metabolic signatures in Ewing sarcoma spheroids.
Article Snippet: .. Single sample gene-set enrichment analyses (ssGSEA) The above-described preprocessed and normalized microarray gene expression data from the EwS cell lines in two different culture conditions without dox treatment (n = 24) were taken as input files and combined with a publicly available and wellcurated gene expression dataset of EwS patient tumors (n = 117; GEO accession code GSE34620)29 generated on GeneChip Human Genome U133 Plus 2.0 Array microarrays (Applied Biosystems, Waltham, MA, USA). ..

Article Title: Refined culture conditions with increased physiological relevance uncover oncogene-dependent metabolic signatures in Ewing sarcoma spheroids
Article Snippet: Before loading the samples on the western blot gel, 20 μg of protein were denaturized at 95°C for 5 min. Then, with a 10% acrylamide gel (Bio-Rad, Munich, Germany) the samples were separated and blotted onto a PVDF membrane (Bio-Rad, Munich, Germany) that was pre-activated in 100% methanol, using a TurboTransfer system (Bio-Rad, Munich, Germany). .. The above-described preprocessed and normalized microarray gene expression data from the EwS cell lines in two different culture conditions without dox treatment ( n = 24) were taken as input files and combined with a publicly available and well-curated gene expression dataset of EwS patient tumors ( n = 117; GEO accession code GSE34620 ) generated on GeneChip Human Genome U133 Plus 2.0 Array microarrays (Applied Biosystems, Waltham, MA, USA). ..

Gene Expression:

Generated:

Single Cell:

Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
Article Snippet: .. Cells were treated with doxycycline as above; after 14 days of DOX treatment, cells were dissociated to single cell suspension and stained with LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Invitrogen, L34976) diluted 1:1000 in PBS for 10 min at room temperature. ..

Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
Article Snippet: .. Cells were treated with doxycycline as above; after 14 days of DOX treatment, cells were dissociated to single cell suspension and stained with LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Invitrogen, L34976 ) diluted 1:1000 in PBS for 10 min at room temperature. .. Cell viability upon ATOH1 KD, was quantified with 20 μL CellTiter-Glo 3D luminescent assay (Promega, G9683) per well, incubated for 30 min to stabilise the signal, before reading luminescence on a FLOUStar Omega plate reader (BMG LABTECH).

Suspension:

Staining:

Article Title: Activation of a branched-chain amino acid rheostat restores replication-dependent hematopoietic stem cell fitness.
Article Snippet: Article Activation of a branched-chain amino acid rheostat restores replication-dependent hematopoietic stem cell fitness

Viability Assay:

Article Title: Matrix type influences embedded patient-derived osteosarcoma organoid invasion and response to treatment
Article Snippet: .. The viability of matrix-free organoids on day 7 and 14 of culture, and embedded organoids at the endpoint of DOX treatment (day 4 embedding), was determined using a fluorescein diacetate (FDA) (Thermo Fisher)/propidium iodide (PI) (Thermo Fisher) cell viability assay. ..

Enzyme-linked Immunosorbent Assay:

Article Title: In Vivo Photoacoustic Monitoring of Stem Cell Location and Apoptosis with Caspase-3-Responsive Nanosensors
Article Snippet: The resulting images were analyzed using Zen Black software (Zeiss). .. The levels of caspase-3 due to AuPD-labeling or DOX treatment were first assessed using a human active caspase-3 ELISA kit according to the protocol provided by the manufacturer (Invitrogen). ..

Article Title: In Vivo Photoacoustic Monitoring of Stem Cell Location and Apoptosis with Caspase-3-Responsive Nanosensors.
Article Snippet: .. The levels of caspase-3 due to AuPDlabeling or DOX treatment were first assessed using a human active caspase-3 ELISA kit according to the protocol provided by the manufacturer (Invitrogen). ..

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